Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with EIF4E2 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Western blot analysis of EIF4E2 (arrow) using rabbit polyclonal EIF4E2 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the EIF4E2 gene.Application
EIF4E2 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | EIF4E2 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHCWBFC |
| IHC:1:10~50 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | EIF4EL3 |
|---|---|
| Alternative Names | EIF4E2 EIF4EL3 Eukaryotic translation initiation factor 4E type 2 Eukaryotic translation initiation factor 4E homologous protein Eukaryotic translation initiation factor 4E-like 3 eIF4E-like protein 4E-LP mRNA cap-binding protein 4EHP mRNA cap-bindi |
| Molecular Weight(MW) | 28362 Da |
| Function | Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures |
| Tissue Specificity | This EIF4E2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the Center region of human EIF4E2. |
| Entrez Gene | 9470 |
|---|
Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with EIF4E2 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Western blot analysis of EIF4E2 (arrow) using rabbit polyclonal EIF4E2 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the EIF4E2 gene.
Application
EIF4E2 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | IHC:1:10~50 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
