Application
A. Western blot analysis on Jurkat cell lysate using anti-Emtk/Itk/Tsk (N-term) Rabbit Monoclonal (Cat. #AJ1259c), dilution 1:5,000Application
B. Immunohistochemical analysis of paraffin-embedded human lymph node using anti- Emtk/Itk/Tsk (N-term) RabMAb (Cat. #AJ1259c).| Product Name | EMTK/ITK/TSK Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ITK, EMT, LYK, Tyrosine-protein kinase ITK/TSK, T-cell-specific kinase;Tyrosine-protein kinase Lyk;Kinase EMT |
| Clonality | Monoclonal |
|---|---|
| Clone Number | Y401 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~5000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | EMT LYK |
|---|---|
| Alternative Names | ITK EMT LYK Tyrosine-protein kinase ITK/TSK T-cell-specific kinase Tyrosine-protein kinase Lyk Kinase EMT |
| Molecular Weight(MW) | 71831 Da |
| Function | Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of ITK to the cell membrane, in the vicinity of the stimulated TCR receptor, where it is phosphorylated by LCK Phosphorylation leads to ITK autophosphorylation and full activation. Once activated, phosphorylates PLCG1, leading to the activation of this lipase and subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. Phosphorylates 2 essential adapter proteins: the linker for activation of T-cells/LAT protein and LCP2. Then, a large number of signaling molecules such as VAV1 are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation |
| Tissue Specificity | T-cell lines and natural killer cell lines. |
| Cellular Localization | Cytoplasm. Note=Localizes in the vicinity of cell surface receptors in the plasma membrane after receptor stimulation |
| Entrez Gene | 3702 |
|---|
Application
A. Western blot analysis on Jurkat cell lysate using anti-Emtk/Itk/Tsk (N-term) Rabbit Monoclonal (Cat. #AJ1259c), dilution 1:5,000
Application
B. Immunohistochemical analysis of paraffin-embedded human lymph node using anti- Emtk/Itk/Tsk (N-term) RabMAb (Cat. #AJ1259c).| Application Notes | WB:1:500~5000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
Data sheet for OM232178