Application
A.Western blot analysis on Hela cell lysate using anti-ERK3 RabMAb(Cat. #AJ1273a), dilution 1:1000.Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-ERK3 RabMAb (Cat. #AJ1273a).| Product Name | Erk3/MAPK6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MAPK6, ERK3, PRKM6, Mitogen-activated protein kinase 6, MAP kinase isoform p97;p97-MAPK |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1720Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ERK3 PRKM6 |
|---|---|
| Alternative Names | MAPK6 ERK3 PRKM6 Mitogen-activated protein kinase 6 MAP kinase isoform p97 p97-MAPK |
| Molecular Weight(MW) | 82681 Da |
| Function | Atypical MAPK protein. Phosphorylates microtubule- associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK3/MAPK6 is phosphorylated at Ser-189 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6. May promote entry in the cell cycle (By similarity) |
| Tissue Specificity | Highest expression in the skeletal muscle, followed by the brain. Also found in heart, placenta, lung, liver, pancreas, kidney and skin fibroblasts |
| Cellular Localization | Cytoplasm (By similarity). Nucleus (By similarity). Note=Translocates to the cytoplasm following interaction with MAPKAPK5 (By similarity) |
| Entrez Gene | 5597 |
|---|
Application
A.Western blot analysis on Hela cell lysate using anti-ERK3 RabMAb(Cat. #AJ1273a), dilution 1:1000.
Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-ERK3 RabMAb (Cat. #AJ1273a).| Application Notes | WB:1:1000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
