Application
Western blot analysis of anti-ErbB3 Pab in mouse brain lysate. ErbB3 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Application
Formalin-fixed and paraffin-embedded human breast carcinoma with ErbB3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of 293 cells using ErbB3 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | E_B3 (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ERBB3; HER3; Receptor tyrosine-protein kinase erbB-3; Proto-oncogene-like protein c-ErbB-3; Tyrosine kinase-type cell surface receptor HER3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HER3 |
|---|---|
| Alternative Names | ERBB3 HER3 Receptor tyrosine-protein kinase erbB-3 Proto-oncogene-like protein c-ErbB-3 Tyrosine kinase-type cell surface receptor HER3 |
| Molecular Weight(MW) | 148098 Da |
| Function | Binds and is activated by neuregulins and NTAK. |
| Tissue Specificity | Epithelial tissues and brain. |
| Cellular Localization | Isoform 1: Cell membrane; Single-pass type I membrane protein |
| Entrez Gene | 2065 |
|---|
Application
Western blot analysis of anti-ErbB3 Pab in mouse brain lysate. ErbB3 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Application
Formalin-fixed and paraffin-embedded human breast carcinoma with ErbB3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of 293 cells using ErbB3 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
