Application
Western blot analysis of FARS2 Antibody (C-term) in WiDr cell line lysates (35ug/lane).FARS2 (arrow) was detected using the purified Pab.Application
FARS2 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FARS2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
FARS2 Antibody (C-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | FARS2 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FARS2; FARS1; Phenylalanine--tRNA ligase, mitochondrial; Phenylalanyl-tRNA synthetase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | FARS1 |
|---|---|
| Alternative Names | FARS2 FARS1 Phenylalanine--tRNA ligase mitochondrial Phenylalanyl-tRNA synthetase |
| Molecular Weight(MW) | 52357 Da |
| Function | Catalyzes direct attachment of p-Tyr (Tyr) to tRNAPhe. Permits also, with a lower efficiency, the attachment of m-Tyr to tRNAPhe, thereby opening the way for delivery of the misacylated tRNA to the ribosome and incorporation of ROS-damaged amino acid into proteins |
| Tissue Specificity | This FARS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 385-415 amino acids from the C-terminal region of human FARS2. |
| Cellular Localization | Mitochondrion matrix (Potential). |
| Entrez Gene | 10667 |
|---|
Application
Western blot analysis of FARS2 Antibody (C-term) in WiDr cell line lysates (35ug/lane).FARS2 (arrow) was detected using the purified Pab.
Application
FARS2 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FARS2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
FARS2 Antibody (C-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
