Application
Western blot analysis of FAS (arrow) using rabbit polyclonal FAS Antibody (pY291) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FAS gene.| Product Name | FAS Antibody (C-term Y291) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FAS; APT1; FAS1; TNFRSF6; Tumor necrosis factor receptor superfamily member 6; Apo-1 antigen; Apoptosis-mediating surface antigen FAS; FASLG receptor; CD_antigen=CD95; Flags: Precursor |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | APT1 FAS1 TNFRSF6 |
|---|---|
| Alternative Names | FAS APT1 FAS1 TNFRSF6 Tumor necrosis factor receptor superfamily member 6 Apo-1 antigen Apoptosis-mediating surface antigen FAS FASLG receptor CD_antigen=CD95 Flags: Precursor |
| Molecular Weight(MW) | 37732 Da |
| Function | Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death- inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS- mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro) |
| Tissue Specificity | Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6 |
| Cellular Localization | Isoform 1: Cell membrane; Single-pass type I membrane protein Isoform 3: Secreted. Isoform 5: Secreted. |
| Entrez Gene | 355 |
|---|---|
| Nucleotide Accession | NP_690610.1 NP_690611.1 NP_000034.1 |
Application
Western blot analysis of FAS (arrow) using rabbit polyclonal FAS Antibody (pY291) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FAS gene.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
