Application
Western blot analysis of FBXL5 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). FBXL5 (arrow) was detected using the purified Pab;Application
Western blot analysis of FBXL5 Antibody (N-term) in mouse heart tissue lysates (35ug/lane). FBXL5 (arrow) was detected using the purified Pab.Application
FBXL5 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FBXL5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
FBXL5 Antibody (N-term) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | FBXL5 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FBXL5; FBL4; FBL5; FLR1; F-box/LRR-repeat protein 5; F-box and leucine-rich repeat protein 5; F-box protein FBL4/FBL5; p45SKP2-like protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | FBL4 FBL5 FLR1 |
|---|---|
| Alternative Names | FBXL5 FBL4 FBL5 FLR1 F-box/LRR-repeat protein 5 F-box and leucine-rich repeat protein 5 F-box protein FBL4/FBL5 p45SKP2-like protein |
| Molecular Weight(MW) | 78555 Da |
| Function | Component of some SCF (SKP1-cullin-F-box) protein ligase complex that plays a central role in iron homeostasis by promoting the ubiquitination and subsequent degradation of IREB2/IRP2. Upon high iron and oxygen level, it specifically recognizes and binds IREB2/IRP2, promoting its ubiquitination and degradation by the proteasome. Promotes ubiquitination and subsequent degradation of DCTN1/p150-glued |
| Tissue Specificity | This FBXL5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 93~123 amino acids from the N-terminal region of human FBXL5. |
| Cellular Localization | Cytoplasm, perinuclear region. |
| Entrez Gene | 26234 |
|---|
Application
Western blot analysis of FBXL5 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). FBXL5 (arrow) was detected using the purified Pab;
Application
Western blot analysis of FBXL5 Antibody (N-term) in mouse heart tissue lysates (35ug/lane). FBXL5 (arrow) was detected using the purified Pab.
Application
FBXL5 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FBXL5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
FBXL5 Antibody (N-term) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
