Application
FKBP8 Antibody (Center) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the FKBP8 antibody detected the FKBP8 protein (arrow).Application
With HepG2 cell line lysate, the resolved proteins were electrophoretically transferred to PVDF membrane and incubated sequentially with primary antibody FKBP38 ( abgent, Cat. AP5990C, 1:1000, 4°C，overnight ) and horseradish peroxidase–conjugated second antibody. After washing, the bound antibody complex was detected using an ECL chemiluminescence reagentand XAR film (Kodak).| Product Name | FKBP8 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | FKBP38 |
|---|---|
| Alternative Names | FKBP8 FKBP38 Peptidyl-prolyl cis-trans isomerase FKBP8 38 kDa FK506-binding protein FK506-binding protein 8 FKBPR38 Rotamase |
| Molecular Weight(MW) | 44562 Da |
| Function | Constitutively inactive PPiase, which becomes active when bound to calmodulin and calcium. Seems to act as a chaperone for BCL2, targets it to the mitochondria and modulates its phosphorylation state. The BCL2/FKBP8/calmodulin/calcium complex probably interferes with the binding of BCL2 to its targets. The active form of FKBP8 may therefore play a role in the regulation of apoptosis |
| Tissue Specificity | Widely expressed. Highest levels seen in the brain |
| Cellular Localization | Mitochondrion membrane; Single-pass membrane protein; Cytoplasmic side (By similarity) |
| Entrez Gene | 23770 |
|---|---|
| Nucleotide Accession | NP_036313.3 |
Application
FKBP8 Antibody (Center) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the FKBP8 antibody detected the FKBP8 protein (arrow).
Application
With HepG2 cell line lysate, the resolved proteins were electrophoretically transferred to PVDF membrane and incubated sequentially with primary antibody FKBP38 ( abgent, Cat. AP5990C, 1:1000, 4°C，overnight ) and horseradish peroxidase–conjugated second antibody. After washing, the bound antibody complex was detected using an ECL chemiluminescence reagentand XAR film (Kodak).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
