Application
FLNA Antibody (Y1046) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the FLNA antibody detected the FLNA protein (arrow).Application
FLNA Antibody (Y1046) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FLNA Antibody (Y1046) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of FLNA Antibody (Y1046)(Cat#AP7770a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).Application
FLNA Antibody (Y1046) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | FLNA Antibody (Y1046) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FLNA; FLN; FLN1; Filamin-A; Actin-binding protein 280; Alpha-filamin; Endothelial actin-binding protein; Filamin-1; Non-muscle filamin |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIFFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | FLN FLN1 |
|---|---|
| Alternative Names | FLNA FLN FLN1 Filamin-A Actin-binding protein 280 Alpha-filamin Endothelial actin-binding protein Filamin-1 Non-muscle filamin |
| Molecular Weight(MW) | 280739 Da |
| Function | Promotes orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Anchors various transmembrane proteins to the actin cytoskeleton and serves as a scaffold for a wide range of cytoplasmic signaling proteins Interaction with FLNA may allow neuroblast migration from the ventricular zone into the cortical plate. Tethers cell surface- localized furin, modulates its rate of internalization and directs its intracellular trafficking (By similarity). Involved in ciliogenesis |
| Tissue Specificity | Ubiquitous. |
| Cellular Localization | Cytoplasm, cell cortex. Cytoplasm, cytoskeleton |
| Entrez Gene | 2316 |
|---|
Application
FLNA Antibody (Y1046) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the FLNA antibody detected the FLNA protein (arrow).
Application
FLNA Antibody (Y1046) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FLNA Antibody (Y1046) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of FLNA Antibody (Y1046)(Cat#AP7770a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Application
FLNA Antibody (Y1046) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
