Application
FOXO3 Antibody (N-term) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the FOXO3 antibody detected the FOXO3 protein (arrow).Application
FOXO3 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FOXO3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | FOXO3 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FOXO3; FKHRL1; FOXO3A; Forkhead box protein O3; AF6q21 protein; Forkhead in rhabdomyosarcoma-like 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | FKHRL1 FOXO3A |
|---|---|
| Alternative Names | FOXO3 FKHRL1 FOXO3A Forkhead box protein O3 AF6q21 protein Forkhead in rhabdomyosarcoma-like 1 |
| Molecular Weight(MW) | 71277 Da |
| Function | Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post- transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation |
| Tissue Specificity | Ubiquitous. |
| Cellular Localization | Cytoplasm, cytosol. Nucleus. Note=Translocates to the nucleus upon oxidative stress and in the absence of survival factors |
| Entrez Gene | 2309 |
|---|---|
| Nucleotide Accession | NP_963853.1 NP_001446.1 |
Application
FOXO3 Antibody (N-term) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the FOXO3 antibody detected the FOXO3 protein (arrow).
Application
FOXO3 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FOXO3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
