Application
A.Western blot analysis on Jurkat cell lysate using anti-FOXO3a Rabbit Monoclonal (Cat. #AJ1305a), dilution 1:5000.Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-FOXO3a RabMAb (Cat. #AJ1305a).| Product Name | FOXO3a Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FOXO3, FKHRL1, FOXO3A, Forkhead box protein O3, Forkhead in rhabdomyosarcoma-like 1;AF6q21 protein |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1949Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:5000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | FKHRL1 FOXO3A |
|---|---|
| Alternative Names | FOXO3 FKHRL1 FOXO3A Forkhead box protein O3 Forkhead in rhabdomyosarcoma-like 1 AF6q21 protein |
| Molecular Weight(MW) | 71277 Da |
| Function | Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post- transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation |
| Tissue Specificity | Ubiquitous. |
| Cellular Localization | Cytoplasm, cytosol. Nucleus. Note=Translocates to the nucleus upon oxidative stress and in the absence of survival factors |
| Entrez Gene | 2309 |
|---|
Application
A.Western blot analysis on Jurkat cell lysate using anti-FOXO3a Rabbit Monoclonal (Cat. #AJ1305a), dilution 1:5000.
Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-FOXO3a RabMAb (Cat. #AJ1305a).| Application Notes | WB:1:5000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
