Application
FREQ Antibody (N-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the FREQ antibody detected the FREQ protein (arrow).Application
FREQ Antibody (N-term) (AP14572a)immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FREQ Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | FREQ Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NCS1; FLUP; FREQ; Neuronal calcium sensor 1; Frequenin homolog; Frequenin-like protein; Frequenin-like ubiquitous protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | FLUP FREQ |
|---|---|
| Alternative Names | NCS1 FLUP FREQ Neuronal calcium sensor 1 Frequenin homolog Frequenin-like protein Frequenin-like ubiquitous protein |
| Molecular Weight(MW) | 21879 Da |
| Function | Neuronal calcium sensor, regulator of G protein-coupled receptor phosphorylation in a calcium dependent manner. Directly regulates GRK1 (RHOK), but not GRK2 to GRK5. Can substitute for calmodulin (By similarity). Stimulates PI4KB kinase activity (By similarity). Involved in long-term synaptic plasticity through its interaction with PICK1 (By similarity). May also play a role in neuron differentiation through inhibition of the activity of N- type voltage-gated calcium channel (By similarity) |
| Tissue Specificity | This FREQ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human FREQ. |
| Cellular Localization | Golgi apparatus, Golgi stack membrane; Peripheral membrane protein. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density (By similarity). Cytoplasm, perinuclear region. Cell membrane; Peripheral membrane protein Note=Associated with Golgi stacks. Post-synaptic densities of dendrites, and in the pre-synaptic nerve terminal at neuromuscular junctions |
| Entrez Gene | 23413 |
|---|---|
| Nucleotide Accession | NP_001122298.1 NP_055101.2 |
Application
FREQ Antibody (N-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the FREQ antibody detected the FREQ protein (arrow).
Application
FREQ Antibody (N-term) (AP14572a)immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FREQ Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
