Application
GAD2 Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the GAD2 antibody detected the GAD2 protein (arrow).Application
GAD2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GAD2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of GAD2 Antibody (Center) with 293 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | GAD2 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | GAD65 |
|---|---|
| Alternative Names | GAD2 GAD65 Glutamate decarboxylase 2 65 kDa glutamic acid decarboxylase Glutamate decarboxylase 65 kDa isoform |
| Molecular Weight(MW) | 65411 Da |
| Function | Catalyzes the production of GABA. |
| Tissue Specificity | This GAD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-145 amino acids from the Central region of human GAD2. |
| Cellular Localization | Cytoplasm, cytosol. Cytoplasmic vesicle. Cell junction, synapse, presynaptic cell membrane; Lipid-anchor Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Note=Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters |
| Entrez Gene | 2572 |
|---|---|
| Nucleotide Accession | NP_001127838.1 NP_000809.1 |
Application
GAD2 Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the GAD2 antibody detected the GAD2 protein (arrow).
Application
GAD2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GAD2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of GAD2 Antibody (Center) with 293 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
