Application
Western blot analysis of GCLM Antibody (C-term) in 293 cell line and mouse kidney tissue lysates (35ug/lane). GCLM (arrow) was detected using the purified Pab.Application
GCLM Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GCLM Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
GCLM Antibody (C-term) flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of GCLM (arrow) using rabbit polyclonal GCLM Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GCLM gene.Application
Confocal immunofluorescent analysis of GCLM Antibody (C-term)(Cat#AP9102b) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | GCLM Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | GLCLR |
|---|---|
| Alternative Names | GCLM GLCLR Glutamate--cysteine ligase regulatory subunit GCS light chain Gamma-ECS regulatory subunit Gamma-glutamylcysteine synthetase regulatory subunit Glutamate--cysteine ligase modifier subunit |
| Molecular Weight(MW) | 30727 Da |
| Tissue Specificity | In all tissues examined. Highest levels in skeletal muscle |
| Entrez Gene | 2730 |
|---|
Application
Western blot analysis of GCLM Antibody (C-term) in 293 cell line and mouse kidney tissue lysates (35ug/lane). GCLM (arrow) was detected using the purified Pab.
Application
GCLM Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GCLM Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
GCLM Antibody (C-term) flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of GCLM (arrow) using rabbit polyclonal GCLM Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GCLM gene.
Application
Confocal immunofluorescent analysis of GCLM Antibody (C-term)(Cat#AP9102b) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
