Application
Immunofluorecence staining of anti-GPC3 Pab (cat# AP6340d) on HepG2 cells. The cells were acetone fixated. Antibody dilution of 1:50. Original magnification 1:400. Data and protocol courtesy of Dr. Mariana Dabeva, Department of Medicine at Albert Einstein College of Medicine.Application
GPC3 Antibody?P544? (cat# AP6340d) western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the GPC3 antibody detected the GPC3 protein (arrow).Application
GPC3 Antibody (P544) (cat# AP6340d) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the GPC3 antibody detected the GPC3 protein (arrow).| Product Name | Glypican 3 (GPC3) Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GPC3; OCI5; Glypican-3; GTR2-2; Intestinal protein OCI-5; MXR7; Secreted glypican-3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFWB |
| IF:1:10~50 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | OCI5 |
|---|---|
| Alternative Names | GPC3 OCI5 Glypican-3 GTR2-2 Intestinal protein OCI-5 MXR7 Secreted glypican-3 |
| Molecular Weight(MW) | 65563 Da |
| Function | Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition |
| Tissue Specificity | Highly expressed in lung, liver and kidney. |
| Cellular Localization | Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side (By similarity) |
| Entrez Gene | 2719 |
|---|
Application
Immunofluorecence staining of anti-GPC3 Pab (cat# AP6340d) on HepG2 cells. The cells were acetone fixated. Antibody dilution of 1:50. Original magnification 1:400. Data and protocol courtesy of Dr. Mariana Dabeva, Department of Medicine at Albert Einstein College of Medicine.
Application
GPC3 Antibody?P544? (cat# AP6340d) western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the GPC3 antibody detected the GPC3 protein (arrow).
Application
GPC3 Antibody (P544) (cat# AP6340d) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the GPC3 antibody detected the GPC3 protein (arrow).| Application Notes | IF:1:10~50 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
