Application
The anti-GPI8 Pab is used in Western blot to detect GPI8 in 293 cell lysate.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | GPI8 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PIGK; GPI8; GPI-anchor transamidase; GPI8 homolog; Phosphatidylinositol-glycan biosynthesis class K protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | GPI8 |
|---|---|
| Alternative Names | PIGK GPI8 GPI-anchor transamidase GPI8 homolog Phosphatidylinositol-glycan biosynthesis class K protein |
| Molecular Weight(MW) | 45252 Da |
| Function | Mediates GPI anchoring in the endoplasmic reticulum, by replacing a protein's C-terminal GPI attachment signal peptide with a pre-assembled GPI. During this transamidation reaction, the GPI transamidase forms a carbonyl intermediate with the substrate protein |
| Tissue Specificity | This GPI8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 45~74 amino acids from the N-terminal region of human GPI8. |
| Cellular Localization | Endoplasmic reticulum membrane; Single-pass type I membrane protein |
| Entrez Gene | 10026 |
|---|
Application
The anti-GPI8 Pab is used in Western blot to detect GPI8 in 293 cell lysate.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
