Application
Western blot analysis of GPT antibody (N-term P101) in HL60 cell line lysates (35ug/lane). GPT (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma with GPT Antibody (N-term P101), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of 293 cells using GPT Antibody (N-term P101)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | GPT Antibody (N-term P101) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GPT; AAT1; GPT1; Alanine aminotransferase 1; Glutamate pyruvate transaminase 1; Glutamic--alanine transaminase 1; Glutamic--pyruvic transaminase 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | AAT1 GPT1 |
|---|---|
| Alternative Names | GPT AAT1 GPT1 Alanine aminotransferase 1 Glutamate pyruvate transaminase 1 Glutamic--alanine transaminase 1 Glutamic--pyruvic transaminase 1 |
| Molecular Weight(MW) | 54637 Da |
| Function | Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles (By similarity) |
| Tissue Specificity | Liver, kidney, heart, and skeletal muscles. Expressed at moderate levels in the adipose tissue |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 2875 |
|---|
Application
Western blot analysis of GPT antibody (N-term P101) in HL60 cell line lysates (35ug/lane). GPT (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma with GPT Antibody (N-term P101), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of 293 cells using GPT Antibody (N-term P101)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
