Application
Western blot analysis of GSTP1 Antibody (Center) in Y79, K562, WIDr, Ramos cell line lysates (35ug/lane). GSTP1 (arrow) was detected using the purified Pab.Application
GSTP1 Antibody (Center) (RB20807) IHC analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GSTP1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
GSTP1 Antibody (Center) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of GSTP1 (arrow) using rabbit polyclonal GSTP1 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GSTP1 gene.Application
Confocal immunofluorescent analysis of GSTP1 Antibody (Center)(Cat#AP9199c) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | GSTP1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GSTP1; FAEES3; GST3; Glutathione S-transferase P; GST class-pi; GSTP1-1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | FAEES3 GST3 |
|---|---|
| Alternative Names | GSTP1 FAEES3 GST3 Glutathione S-transferase P GST class-pi GSTP1-1 |
| Molecular Weight(MW) | 23356 Da |
| Function | Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Regulates negatively CDK5 activity via p25/p35 translocation to prevent neurodegeneration |
| Tissue Specificity | This GSTP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104~134 amino acids from the Center region of human GSTP1. |
| Cellular Localization | Cytoplasm. Mitochondrion. Nucleus. Note=The 83 N-terminal amino-acids function as un uncleaved transit peptide, and arginine residues within it are crucial for motochondrial localization |
| Entrez Gene | 2950 |
|---|
Application
Western blot analysis of GSTP1 Antibody (Center) in Y79, K562, WIDr, Ramos cell line lysates (35ug/lane). GSTP1 (arrow) was detected using the purified Pab.
Application
GSTP1 Antibody (Center) (RB20807) IHC analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GSTP1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
GSTP1 Antibody (Center) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of GSTP1 (arrow) using rabbit polyclonal GSTP1 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GSTP1 gene.
Application
Confocal immunofluorescent analysis of GSTP1 Antibody (Center)(Cat#AP9199c) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
