Application
Western blot analysis of HADHA Antibody (C-term) in A2058(lane 1), A375(lane 2), NCI-H460(lane 3), Ramos(lane 4) cell line lysates (35ug/lane). HADHA (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HADHA Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
HADHA Antibody (C-term) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | HADHA Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HADHA; HADH; Trifunctional enzyme subunit alpha, mitochondrial; 78 kDa gastrin-binding protein; TP-alpha; Long-chain enoyl-CoA hydratase; Long chain 3-hydroxyacyl-CoA dehydrogenase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HADH |
|---|---|
| Alternative Names | HADHA HADH Trifunctional enzyme subunit alpha mitochondrial 78 kDa gastrin-binding protein TP-alpha Long-chain enoyl-CoA hydratase Long chain 3-hydroxyacyl-CoA dehydrogenase |
| Molecular Weight(MW) | 83000 Da |
| Function | Bifunctional subunit. |
| Tissue Specificity | This HADHA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 734~763 amino acids from the C-terminal region of human HADHA. |
| Cellular Localization | Mitochondrion. |
| Entrez Gene | 3030 |
|---|
Application
Western blot analysis of HADHA Antibody (C-term) in A2058(lane 1), A375(lane 2), NCI-H460(lane 3), Ramos(lane 4) cell line lysates (35ug/lane). HADHA (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HADHA Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
HADHA Antibody (C-term) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
