Application
Western blot analysis of anti-HDGF Pab in Hela cell line lysates (35ug/lane). HDGF(arrow) was detected using the purified Pab (1:60 dilution).Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HDGF antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of HDGF Antibody (C-term)(Cat#AP6375b) with SK-BR-3 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).| Product Name | HDGF Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HDGF; HMG1L2; Hepatoma-derived growth factor; High mobility group protein 1-like 2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HMG1L2 |
|---|---|
| Alternative Names | HDGF HMG1L2 Hepatoma-derived growth factor High mobility group protein 1-like 2 |
| Molecular Weight(MW) | 26788 Da |
| Function | Heparin-binding protein, with mitogenic activity for fibroblasts. Acts as a transcriptional repressor |
| Tissue Specificity | Ubiquitous. |
| Cellular Localization | Cytoplasm. Nucleus. |
| Entrez Gene | 3068 |
|---|
Application
Western blot analysis of anti-HDGF Pab in Hela cell line lysates (35ug/lane). HDGF(arrow) was detected using the purified Pab (1:60 dilution).
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HDGF antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of HDGF Antibody (C-term)(Cat#AP6375b) with SK-BR-3 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
