Application
Western blot analysis of anti-HES1 Antibody (N-term T24) (RB13980) in HL60 cell line lysates (35ug/lane). HES1 (arrow) was detected using the purified Pab (1:60 dilution).Application
Confocal immunofluorescent analysis of HES1 Antibody (N-term T24)(Cat#AP6276a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).Application
HES1 Antibody (N-term T24) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | HES1 Antibody (N-term T24) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HES1; BHLHB39; HL; HRY; Transcription factor HES-1; Class B basic helix-loop-helix protein 39; Hairy and enhancer of split 1; Hairy homolog; Hairy-like protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIFFC |
| WB:1:50~100 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BHLHB39 HL HRY |
|---|---|
| Alternative Names | HES1 BHLHB39 HL HRY Transcription factor HES-1 Class B basic helix-loop-helix protein 39 Hairy and enhancer of split 1 Hairy homolog Hairy-like protein |
| Molecular Weight(MW) | 29541 Da |
| Function | Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity (By similarity). May play a role in a functional FA core complex response to DNA cross-link damage, being required for the stability and nuclear localization of FA core complex proteins, as well as for FANCD2 monoubiquitination in response to DNA damage |
| Tissue Specificity | This HES1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16~46 amino acids from the N-terminal region of human HES1. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 3280 |
|---|
Application
Western blot analysis of anti-HES1 Antibody (N-term T24) (RB13980) in HL60 cell line lysates (35ug/lane). HES1 (arrow) was detected using the purified Pab (1:60 dilution).
Application
Confocal immunofluorescent analysis of HES1 Antibody (N-term T24)(Cat#AP6276a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Application
HES1 Antibody (N-term T24) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
