Application
A. Western blot analysis on Ramos + CoCl2 lysate using anti-HIF-1 alpha RabMAb (Cat. #AJ1345a), dilution 1:2000Application
B. Immunohistochemical staining of paraffin-embedded human liver carcinoma using anti-HIF-1 alpha RabMAb (Cat. #AJ1345a).| Product Name | HIF-1 alpha Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HIF1A, MOP1, Hypoxia-inducible factor 1 alpha, ARNT-interacting protein;Member of PAS protein 1;Basic-helix-loop-helix-PAS protein MOP1 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1215Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000~2000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BHLHE78 MOP1 PASD8 |
|---|---|
| Alternative Names | HIF1A MOP1 Hypoxia-inducible factor 1 alpha ARNT-interacting protein Member of PAS protein 1 Basic-helix-loop-helix-PAS protein MOP1 |
| Molecular Weight(MW) | 92670 Da |
| Function | Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia |
| Tissue Specificity | Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors |
| Cellular Localization | Cytoplasm. Nucleus. Note=Cytoplasmic in normoxia, nuclear translocation in response to hypoxia Colocalizes with SUMO1 in the nucleus, under hypoxia |
| Entrez Gene | 3091 |
|---|
Application
A. Western blot analysis on Ramos + CoCl2 lysate using anti-HIF-1 alpha RabMAb (Cat. #AJ1345a), dilution 1:2000
Application
B. Immunohistochemical staining of paraffin-embedded human liver carcinoma using anti-HIF-1 alpha RabMAb (Cat. #AJ1345a).| Application Notes | WB:1:1000~2000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
