Application
HMGN1 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the HMGN1 antibody detected the HMGN1 protein (arrow).Application
Western blot analysis of HMGN1 (arrow) using rabbit polyclonal HMGN1 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HMGN1 gene.Application
HMGN1 Antibody (N-term) (AP14219a)immunohistochemistry analysis in formalin fixed and paraffin embedded human skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HMGN1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | HMGN1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HMG14 |
|---|---|
| Alternative Names | HMGN1 HMG14 Non-histone chromosomal protein HMG-14 High mobility group nucleosome-binding domain-containing protein 1 |
| Molecular Weight(MW) | 10659 Da |
| Function | Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer May be involved in the process which maintains transcribable genes in an unique chromatin conformation. Inhibits the phosphorylation of nucleosomal histones H3 and H2A by RPS6KA5/MSK1 and RPS6KA3/RSK2 (By similarity) |
| Tissue Specificity | This HMGN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human HMGN1. |
| Cellular Localization | Nucleus. Cytoplasm. Note=Cytoplasmic enrichment upon phosphorylation. The RNA edited version localizes to the nucleus |
| Entrez Gene | 3150 |
|---|---|
| Nucleotide Accession | NP_004956.5 |
Application
HMGN1 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the HMGN1 antibody detected the HMGN1 protein (arrow).
Application
Western blot analysis of HMGN1 (arrow) using rabbit polyclonal HMGN1 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HMGN1 gene.
Application
HMGN1 Antibody (N-term) (AP14219a)immunohistochemistry analysis in formalin fixed and paraffin embedded human skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HMGN1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
