Application
HNRPAB Antibody (N-term) western blot analysis in A375, T47D cell line lysates (35ug/lane).This demonstrates the HNRPAB antibody detected the HNRPAB protein (arrow).Application
HNRPAB Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HNRPAB Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Fluorescent confocal image of MCF-7 cell stained with HNRPAB Antibody (N-term)(Cat#AP5522a).MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HNRPAB primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). HNRPAB immunoreactivity is localized to Nucleus significantly.| Product Name | HNRPAB Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HNRNPAB; ABBP1; HNRPAB; Heterogeneous nuclear ribonucleoprotein A/B; APOBEC1-binding protein 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ABBP1 HNRPAB |
|---|---|
| Alternative Names | HNRNPAB ABBP1 HNRPAB Heterogeneous nuclear ribonucleoprotein A/B APOBEC1-binding protein 1 |
| Molecular Weight(MW) | 36225 Da |
| Function | Binds single-stranded RNA. Has a high affinity for G- rich and U-rich regions of hnRNA. Also binds to APOB mRNA transcripts around the RNA editing site |
| Tissue Specificity | Ubiquitous. |
| Cellular Localization | Nucleus. Cytoplasm. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs |
Application
HNRPAB Antibody (N-term) western blot analysis in A375, T47D cell line lysates (35ug/lane).This demonstrates the HNRPAB antibody detected the HNRPAB protein (arrow).
Application
HNRPAB Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HNRPAB Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Fluorescent confocal image of MCF-7 cell stained with HNRPAB Antibody (N-term)(Cat#AP5522a).MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HNRPAB primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). HNRPAB immunoreactivity is localized to Nucleus significantly.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
