Application
Western blot analysis of HNRPF antibody (Center) in HepG2, Hela, K562 and A375 cell line lysates (35ug/lane). HNRPF (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human brain tissue with HNRPF Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | HNRPF Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HNRPF |
|---|---|
| Alternative Names | HNRNPF HNRPF Heterogeneous nuclear ribonucleoprotein F Nucleolin-like protein mcs94-1 Heterogeneous nuclear ribonucleoprotein F N-terminally processed |
| Molecular Weight(MW) | 45672 Da |
| Function | Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Plays a role in the regulation of alternative splicing events. Binds G-rich sequences in pre-mRNAs and keeps target RNA in an unfolded state |
| Tissue Specificity | Expressed ubiquitously. |
| Cellular Localization | Nucleus, nucleoplasm. |
| Entrez Gene | 3185 |
|---|
Application
Western blot analysis of HNRPF antibody (Center) in HepG2, Hela, K562 and A375 cell line lysates (35ug/lane). HNRPF (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human brain tissue with HNRPF Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
