Application
Western blot analysis of HNRPL Antibody (Center) in HepG2, Jurkat cell line lysates (35ug/lane). HNRPL (arrow) was detected using the purified Pab.Application
Western blot analysis of HNRPL (arrow) using rabbit polyclonal HNRPL Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HNRPL gene (Lane 2) .Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with HNRPL Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
HNRPL Antibody (Center) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | HNRPL Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HNRNPL; HNRPL; Heterogeneous nuclear ribonucleoprotein L |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HNRPL |
|---|---|
| Alternative Names | HNRNPL HNRPL Heterogeneous nuclear ribonucleoprotein L |
| Molecular Weight(MW) | 64133 Da |
| Function | This protein is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Is associated with most nascent transcripts including those of the landmark giant loops of amphibian lampbrush chromosomes. Associates, together with APEX1, to the negative calcium responsive element (nCaRE) B2 of the APEX2 promoter |
| Tissue Specificity | This HNRPL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 256~285 amino acids from the Center region of human HNRPL. |
| Cellular Localization | Nucleus, nucleoplasm. Cytoplasm. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs |
| Entrez Gene | 3191 |
|---|
Application
Western blot analysis of HNRPL Antibody (Center) in HepG2, Jurkat cell line lysates (35ug/lane). HNRPL (arrow) was detected using the purified Pab.
Application
Western blot analysis of HNRPL (arrow) using rabbit polyclonal HNRPL Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HNRPL gene (Lane 2) .
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with HNRPL Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
HNRPL Antibody (Center) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
