Application
Western blot analysis of HPRT1 antibody (C-term) in Hela cell line lysates (35ug/lane). HPRT1 (arrow) was detected using the purified Pab.Application
Western blot analysis of HPRT1 (arrow) using rabbit polyclonal HPRT1 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HPRT1 gene.Application
HPRT1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HPRT1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
HPRT1 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | HPRT1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HPRT1; HPRT; Hypoxanthine-guanine phosphoribosyltransferase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HPRT |
|---|---|
| Alternative Names | HPRT1 HPRT Hypoxanthine-guanine phosphoribosyltransferase |
| Molecular Weight(MW) | 24579 Da |
| Function | Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5- phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway |
| Tissue Specificity | This HPRT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 157~186 amino acids from the C-terminal region of human HPRT1. |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 3251 |
|---|
Application
Western blot analysis of HPRT1 antibody (C-term) in Hela cell line lysates (35ug/lane). HPRT1 (arrow) was detected using the purified Pab.
Application
Western blot analysis of HPRT1 (arrow) using rabbit polyclonal HPRT1 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HPRT1 gene.
Application
HPRT1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HPRT1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
HPRT1 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
