Application
Western blot analysis of anti-HS2ST1 Antibody (Center) Pab in Ramos and CEM cell line lysates. HS2ST1(arrow) was detected using the purified Pab.Application
Western blot analysis of anti-HS2ST1 Antibody (Center) Pab in 293 cell line lysates transiently transfected with the HS2ST1 gene (2ug/lane). HS2ST1(arrow) was detected using the purified Pab (1:60 dilution).Application
Western blot analysis of anti-HS2ST1 Antibody (Center) Pab in 293 cell line lysates transiently transfected with the HS2ST1 gene (2ug/lane). HS2ST1 Antibody (Center)(arrow) was detected using the purified Pab (1:60 dilution).Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with HS2ST1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | HS2ST1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HS2ST1; HS2ST; KIAA0448; Heparan sulfate 2-O-sulfotransferase 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HS2ST KIAA0448 |
|---|---|
| Alternative Names | HS2ST1 HS2ST KIAA0448 Heparan sulfate 2-O-sulfotransferase 1 |
| Molecular Weight(MW) | 41881 Da |
| Function | Catalyzes the transfer of sulfate to the C2-position of selected hexuronic acid residues within the maturing heparan sulfate (HS). 2-O-sulfation within HS, particularly of iduronate residues, is essential for HS to participate in a variety of high- affinity ligand-binding interactions and signaling processes Mediates 2-O-sulfation of both L-iduronyl and D-glucuronyl residues (By similarity) |
| Tissue Specificity | This HS2ST1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 178~207 amino acids from the center region of human HS2ST1 Antibody. |
| Cellular Localization | Golgi apparatus membrane; Single-pass type II membrane protein (By similarity) |
| Entrez Gene | 9653 |
|---|
Application
Western blot analysis of anti-HS2ST1 Antibody (Center) Pab in Ramos and CEM cell line lysates. HS2ST1(arrow) was detected using the purified Pab.
Application
Western blot analysis of anti-HS2ST1 Antibody (Center) Pab in 293 cell line lysates transiently transfected with the HS2ST1 gene (2ug/lane). HS2ST1(arrow) was detected using the purified Pab (1:60 dilution).
Application
Western blot analysis of anti-HS2ST1 Antibody (Center) Pab in 293 cell line lysates transiently transfected with the HS2ST1 gene (2ug/lane). HS2ST1 Antibody (Center)(arrow) was detected using the purified Pab (1:60 dilution).
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with HS2ST1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
