Application
Western blot analysis of HSD3B2 Antibody (N-term) in CEM cell line lysates (35ug/lane). HSD3B2 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human testis reacted with HSD3B2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | HSD3B2 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HSD3B2; HSDB3B; 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2; 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type II; 3-beta-HSD adrenal and gonadal type; 3-beta-hydroxy-Delta(5)-steroid dehydrogenase; 3-beta-hydroxy-5-ene st |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HSDB3B |
|---|---|
| Alternative Names | HSD3B2 HSDB3B 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type II 3-beta-HSD adrenal and gonadal type 3-beta-hydroxy-Delta(5)-steroid dehydrogenase 3-beta-hydroxy-5-ene st |
| Molecular Weight(MW) | 42052 Da |
| Function | 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids |
| Tissue Specificity | Expressed in adrenal gland, testis and ovary. |
| Cellular Localization | Endoplasmic reticulum membrane; Single-pass membrane protein. Mitochondrion membrane; Single-pass membrane protein |
| Entrez Gene | 3284 |
|---|
Application
Western blot analysis of HSD3B2 Antibody (N-term) in CEM cell line lysates (35ug/lane). HSD3B2 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human testis reacted with HSD3B2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
