Application
Western blot analysis of HSP27 antibody (Center) (Cat.# AP7199e) in MCF-7 cell line lysates (35ug/lane). HSP27 (arrow) was detected using the purified Pab.Application
Hsp 27 Antibody (Center) (Cat.# AP7199e) IHC analysis in formalin fixed and paraffin embedded human Lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Hsp 27 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | HSP27 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HSPB1; HSP27; HSP28; Heat shock protein beta-1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; Stress-responsive protein 27 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HSP27 HSP28 |
|---|---|
| Alternative Names | HSPB1 HSP27 HSP28 Heat shock protein beta-1 28 kDa heat shock protein Estrogen-regulated 24 kDa protein Heat shock 27 kDa protein Stress-responsive protein 27 |
| Molecular Weight(MW) | 22783 Da |
| Function | Involved in stress resistance and actin organization. |
| Tissue Specificity | Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle |
| Cellular Localization | Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. Note=Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles |
| Entrez Gene | 3315 |
|---|
Application
Western blot analysis of HSP27 antibody (Center) (Cat.# AP7199e) in MCF-7 cell line lysates (35ug/lane). HSP27 (arrow) was detected using the purified Pab.
Application
Hsp 27 Antibody (Center) (Cat.# AP7199e) IHC analysis in formalin fixed and paraffin embedded human Lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Hsp 27 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
