Application
A. Western blot analysis on Hela cell lysate using anti-Phospho-HSP27 (pS82) RabMAb (Cat. #AJ1378d), 1:5,000 dilution. Cells were either (1) untreated or (2) treated at 42 °C.Application
B. Immunohistochemical analysis of paraffin-embedded urinary bladder carcinoma using anti-Phospho-HSP27 (pS82) RabMAb (Cat. #AJ1378d).Application
C. Immunofluorescent staining of HSP relocating to nucleus upon heat shock in HeLa cells using anti-Phospho-HSP27 (pS82) RabMAb (Cat. #AJ1378d).| Product Name | HSP27 Antibody Phospho (pS82) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HSPB1, HSP27, HSP28, Heat shock protein beta-1, Estrogen-regulated 24 kDa protein;28 kDa heat shock protein |
| Modification | p-S82 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | E118 |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HSP27 HSP28 |
|---|---|
| Alternative Names | HSPB1 HSP27 HSP28 Heat shock protein beta-1 Estrogen-regulated 24 kDa protein 28 kDa heat shock protein |
| Molecular Weight(MW) | 22783 Da |
| Function | Involved in stress resistance and actin organization. |
| Tissue Specificity | Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle |
| Cellular Localization | Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. Note=Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles |
| Entrez Gene | 3315 |
|---|
Application
A. Western blot analysis on Hela cell lysate using anti-Phospho-HSP27 (pS82) RabMAb (Cat. #AJ1378d), 1:5,000 dilution. Cells were either (1) untreated or (2) treated at 42 °C.
Application
B. Immunohistochemical analysis of paraffin-embedded urinary bladder carcinoma using anti-Phospho-HSP27 (pS82) RabMAb (Cat. #AJ1378d).
Application
C. Immunofluorescent staining of HSP relocating to nucleus upon heat shock in HeLa cells using anti-Phospho-HSP27 (pS82) RabMAb (Cat. #AJ1378d).| Application Notes | WB:1:500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
