Application
HSP90B1 Antibody (N-term) western blot analysis in HepG2, CHO, K562, Hela cell line and mouse liver tissue lysates (35ug/lane).This demonstrates the HSP90B1 antibody detected the HSP90B1 protein (arrow).Application
HSP90B1 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HSP90B1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
HSP90B1 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of HSP90B1 Antibody (N-term)(Cat#AP4899a) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | HSP90B1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HSP90B1; GRP94; TRA1; Endoplasmin; 94 kDa glucose-regulated protein; Heat shock protein 90 kDa beta member 1; Tumor rejection antigen 1; gp96 homolog |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | GRP94 TRA1 |
|---|---|
| Alternative Names | HSP90B1 GRP94 TRA1 Endoplasmin 94 kDa glucose-regulated protein Heat shock protein 90 kDa beta member 1 Tumor rejection antigen 1 gp96 homolog |
| Molecular Weight(MW) | 92469 Da |
| Function | Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors (By similarity). Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity |
| Tissue Specificity | This HSP90B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between amino acids 20-50 from the N-terminal region of human HSP90B1. |
| Cellular Localization | Endoplasmic reticulum lumen. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV |
| Entrez Gene | 7184 |
|---|
Application
HSP90B1 Antibody (N-term) western blot analysis in HepG2, CHO, K562, Hela cell line and mouse liver tissue lysates (35ug/lane).This demonstrates the HSP90B1 antibody detected the HSP90B1 protein (arrow).
Application
HSP90B1 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HSP90B1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
HSP90B1 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of HSP90B1 Antibody (N-term)(Cat#AP4899a) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
