Application
ID1 Antibody (Center) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the ID1 antibody detected the ID1 protein (arrow).Application
ID1 Antibody (Center) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the ID1(PEI 1:100) antibody detected the ID1 protein (arrow).Application
ID1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ID1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of ID1 Antibody (Center)(Cat#AP11717c) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).Application
ID1 Antibody (Center) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ID1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ID1; BHLHB24; ID; DNA-binding protein inhibitor ID-1; Class B basic helix-loop-helix protein 24; Inhibitor of DNA binding 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIFFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BHLHB24 ID |
|---|---|
| Alternative Names | ID1 BHLHB24 ID DNA-binding protein inhibitor ID-1 Class B basic helix-loop-helix protein 24 Inhibitor of DNA binding 1 |
| Molecular Weight(MW) | 16133 Da |
| Function | ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding |
| Tissue Specificity | This ID1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 73-101 amino acids from the Central region of human ID1. |
| Cellular Localization | Cytoplasm (By similarity). Nucleus. |
| Entrez Gene | 3397 |
|---|---|
| Nucleotide Accession | NP_851998.1 NP_002156.2 |
Application
ID1 Antibody (Center) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the ID1 antibody detected the ID1 protein (arrow).
Application
ID1 Antibody (Center) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the ID1(PEI 1:100) antibody detected the ID1 protein (arrow).
Application
ID1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ID1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of ID1 Antibody (Center)(Cat#AP11717c) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Application
ID1 Antibody (Center) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
