Application
A. Western blot analysis on Daudi cell lysate using anti-IKK-alpha (N-term) RabMAb (Cat. #AJ1390a) 1:50,000 dilutionApplication
B. Immunohistochemical analysis of paraffin-embedded human stomach carcinoma using anti-IKK alpha (N-term) RabMAb (Cat. #AJ1390a).| Product Name | IKK alpha Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CHUK, IKKA, TCF16, Inhibitor of nuclear factor kappa-B kinase subunit alpha, Conserved helix-loop-helix ubiquitous kinase;Nuclear factor NF-kappa-B inhibitor kinase alpha |
| Clonality | Monoclonal |
|---|---|
| Clone Number | Y463 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | IKKA TCF16 |
|---|---|
| Alternative Names | CHUK IKKA TCF16 Inhibitor of nuclear factor kappa-B kinase subunit alpha Conserved helix-loop-helix ubiquitous kinase Nuclear factor NF-kappa-B inhibitor kinase alpha |
| Molecular Weight(MW) | 84640 Da |
| Function | Serine kinase that plays an essential role in the NF- kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on serine residues These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Negatively regulates the pathway by phosphorylating the scaffold protein TAXBP1 and thus promoting the assembly of the A20/TNFAIP3 ubiquitin-editing complex (composed of A20/TNFAIP3, TAX1BP1, and the E3 ligases ITCH and RNF11). Therefore, CHUK plays a key role in the negative feedback of NF-kappa-B canonical signaling to limit inflammatory gene activation. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa- B RelB-p52 complexes. In turn, these complexes regulate genes encoding molecules involved in B-cell survival and lymphoid organogenesis. Participates also in the negative feedback of the non-canonical NF-kappa-B signaling pathway by phosphorylating and destabilizing MAP3K14/NIK. Within the nucleus, phosphorylates CREBBP and consequently increases both its transcriptional and histone acetyltransferase activities. Modulates chromatin accessibility at NF-kappa-B-responsive promoters by phosphorylating histones H3 at 'Ser-10' that are subsequently acetylated at 'Lys-14' by CREBBP. Additionally, phosphorylates the CREBBP-interacting protein NCOA3 |
| Tissue Specificity | Widely expressed. |
| Cellular Localization | Cytoplasm. Nucleus. Note=Shuttles between the cytoplasm and the nucleus |
| Entrez Gene | 1147 |
|---|
Application
A. Western blot analysis on Daudi cell lysate using anti-IKK-alpha (N-term) RabMAb (Cat. #AJ1390a) 1:50,000 dilution
Application
B. Immunohistochemical analysis of paraffin-embedded human stomach carcinoma using anti-IKK alpha (N-term) RabMAb (Cat. #AJ1390a).| Application Notes | WB:1:1000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
