Application
IKZF1 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the IKZF1 antibody detected the IKZF1 protein (arrow).Application
IKZF1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IKZF1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
IKZF1 Antibody (C-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | IKZF1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IKZF1; IK1; IKAROS; LYF1; ZNFN1A1; DNA-binding protein Ikaros; Ikaros family zinc finger protein 1; Lymphoid transcription factor LyF-1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | IK1 IKAROS LYF1 ZNFN1A1 |
|---|---|
| Alternative Names | IKZF1 IK1 IKAROS LYF1 ZNFN1A1 DNA-binding protein Ikaros Ikaros family zinc finger protein 1 Lymphoid transcription factor LyF-1 |
| Molecular Weight(MW) | 57528 Da |
| Function | Transcription regulator of hematopoietic cell differentiation. Binds gamma-satellite DNA. Binds with higher affinity to gamma satellite A. Plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. Repressor of the TDT (terminal deoxynucleotidyltransferase) gene during thymocyte differentiation. Regulates transcription through association with both HDAC-dependent and HDAC-independent complexes. Targets the 2 chromatin-remodeling complexes, NuRD and BAF (SWI/SNF), in a single complex (PYR complex), to the beta-globin locus in adult erythrocytes. Increases normal apoptosis in adult erythroid cells Confers early temporal competence to retinal progenitor cells (RPCs) (By similarity) |
| Tissue Specificity | Abundantly expressed in thymus, spleen and peripheral blood Leukocytes and lymph nodes. Lower expression in bone marrow and small intestine |
| Cellular Localization | Nucleus. Note=In resting lymphocytes, distributed diffusely throughout the nucleus. Localizes to pericentromeric heterochromatin in proliferating cells. This localization requires DNA binding which is regulated by phosphorylation / dephosphorylation events (By similarity) Isoform Ik6: Cytoplasm (By similarity). |
| Entrez Gene | 10320 |
|---|---|
| Nucleotide Accession | NP_006051.1 |
Application
IKZF1 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the IKZF1 antibody detected the IKZF1 protein (arrow).
Application
IKZF1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IKZF1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
IKZF1 Antibody (C-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
