Application
IL6 Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the IL6 antibody detected the IL6 protein (arrow).Application
IL6 Antibody (Center) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
IL6 Antibody (Center) (AP12474c)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IL6 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | IL6 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IL6; IFNB2; Interleukin-6; B-cell stimulatory factor 2; CTL differentiation factor; Hybridoma growth factor; Interferon beta-2 |
| Conjugation | Agarose |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FCIHCWB |
| WB:1:100~500 FC |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | IFNB2 |
|---|---|
| Alternative Names | IL6 IFNB2 Interleukin-6 B-cell stimulatory factor 2 CTL differentiation factor Hybridoma growth factor Interferon beta-2 |
| Molecular Weight(MW) | 23718 Da |
| Function | Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig- secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance |
| Tissue Specificity | This IL6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-55 amino acids from the Central region of human IL6. |
| Cellular Localization | Secreted. |
| Entrez Gene | 3569 |
|---|---|
| Nucleotide Accession | NP_000591.1 |
Application
IL6 Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the IL6 antibody detected the IL6 protein (arrow).
Application
IL6 Antibody (Center) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
IL6 Antibody (Center) (AP12474c)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IL6 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
