Application
Western blot analysis of ILF3 Antibody (Center) in A2058 cell line lysates (35ug/lane). ILF3 (arrow) was detected using the purified Pab.Application
ILF3 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ILF3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | ILF3 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ILF3; DRBF; MPHOSPH4; NF90; Interleukin enhancer-binding factor 3; Double-stranded RNA-binding protein 76; M-phase phosphoprotein 4; Nuclear factor associated with dsRNA; Nuclear factor of activated T-cells 90 kDa; Translational control protein 80 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | DRBF MPHOSPH4 NF90 |
|---|---|
| Alternative Names | ILF3 DRBF MPHOSPH4 NF90 Interleukin enhancer-binding factor 3 Double-stranded RNA-binding protein 76 M-phase phosphoprotein 4 Nuclear factor associated with dsRNA Nuclear factor of activated T-cells 90 kDa Translational control protein 80 |
| Molecular Weight(MW) | 95338 Da |
| Function | May facilitate double-stranded RNA-regulated gene expression at the level of post-transcription. Can act as a translation inhibitory protein which binds to coding sequences of acid beta-glucosidase (GCase) and other mRNAs and functions at the initiation phase of GCase mRNA translation, probably by inhibiting its binding to polysomes. Can regulate protein arginine N- methyltransferase 1 activity. May regulate transcription of the IL2 gene during T-cell activation. Can promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA |
| Tissue Specificity | Ubiquitous. |
| Cellular Localization | Nucleus, nucleolus. Cytoplasm. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs |
| Entrez Gene | 3609 |
|---|
Application
Western blot analysis of ILF3 Antibody (Center) in A2058 cell line lysates (35ug/lane). ILF3 (arrow) was detected using the purified Pab.
Application
ILF3 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ILF3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
