Application
Western blot analysis on (A) THP-1 treated with TPA, (B) HUVEC, (C) human spleen and (D) human plasma lysates using anti-Integrin beta-3 (CD61 antigen) RabMAb (Cat. #AJ1408d).| Product Name | Integrin beta-3 (CD61) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ITGB3, GP3A, Integrin beta-3, Platelet membrane glycoprotein IIIa |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR2342 |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | GP3A |
|---|---|
| Alternative Names | ITGB3 GP3A Integrin beta-3 Platelet membrane glycoprotein IIIa |
| Molecular Weight(MW) | 87058 Da |
| Function | Integrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha- IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions |
| Tissue Specificity | Isoform beta-3A and isoform beta-3C are widely expressed. Isoform beta-3A is specifically expressed in osteoblast cells; isoform beta-3C is specifically expressed in prostate and testis |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
| Entrez Gene | 3690 |
|---|
Application
Western blot analysis on (A) THP-1 treated with TPA, (B) HUVEC, (C) human spleen and (D) human plasma lysates using anti-Integrin beta-3 (CD61 antigen) RabMAb (Cat. #AJ1408d).| Application Notes | WB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
