Application
IPO9 Antibody (N-term) western blot analysis in mouse Neuro-2a cell line lysates (15ug/lane).This demonstrates the IPO9 antibody detected the IPO9 protein (arrow).Application
IPO9 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IPO9 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
IPO9 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | IPO9 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IPO9; IMP9; KIAA1192; RANBP9; Importin-9; Ran-binding protein 9 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | IMP9 KIAA1192 RANBP9 |
|---|---|
| Alternative Names | IPO9 IMP9 KIAA1192 RANBP9 Importin-9 Ran-binding protein 9 |
| Molecular Weight(MW) | 115963 Da |
| Function | Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of H2B histone (By similarity), RPS7 and RPL18A. Prevents the cytoplasmic aggregation of RPS7 and RPL18A by shielding exposed basic domains May also import H2A, H3, H4 histones (By similarity), RPL4 and RPL6 |
| Tissue Specificity | This IPO9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 86-115 amino acids from the N-terminal region of human IPO9. |
| Cellular Localization | Cytoplasm (By similarity). Nucleus (By similarity) |
| Entrez Gene | 55705 |
|---|---|
| Nucleotide Accession | NP_060555.2 |
Application
IPO9 Antibody (N-term) western blot analysis in mouse Neuro-2a cell line lysates (15ug/lane).This demonstrates the IPO9 antibody detected the IPO9 protein (arrow).
Application
IPO9 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IPO9 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
IPO9 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
