Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with JHDM1a/FBXL11 antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Western blot analysis of JHDM1a/FBXL11 (arrow) using rabbit polyclonal JHDM1a/FBXL11 Antibody (Center).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the JHDM1a/FBXL11 gene (Lane 2) (Origene Technologies).Application
JHDM1a/FBXL11 Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the JHDM1a/FBXL11 antibody detected the JHDM1a/FBXL11 protein (arrow).| Product Name | JHDM1a/FBXL11 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KDM2A; CXXC8; FBL7; FBXL11; JHDM1A; KIAA1004; Lysine-specific demethylase 2A; CXXC-type zinc finger protein 8; F-box and leucine-rich repeat protein 11; F-box protein FBL7; F-box protein Lilina; F-box/LRR-repeat protein 11; JmjC domain-containing histone d |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| IHC:1:10~50 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CXXC8 FBL7 FBXL11 JHDM1A KIAA1004 |
|---|---|
| Alternative Names | KDM2A CXXC8 FBL7 FBXL11 JHDM1A KIAA1004 Lysine-specific demethylase 2A CXXC-type zinc finger protein 8 F-box and leucine-rich repeat protein 11 F-box protein FBL7 F-box protein Lilina F-box/LRR-repeat protein 11 JmjC domain-containing histone d |
| Molecular Weight(MW) | 132793 Da |
| Function | Histone demethylase that specifically demethylates 'Lys- 36' of histone H3, thereby playing a central role in histone code Preferentially demethylates dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylated H3 'Lys- 36'. May also recognize and bind to some phosphorylated proteins and promote their ubiquitination and degradation. Required to maintain the heterochromatic state. Associates with centromeres and represses transcription of small non-coding RNAs that are encoded by the clusters of satellite repeats at the centromere Required to sustain centromeric integrity and genomic stability, particularly during mitosis |
| Tissue Specificity | Widely expressed, with highest levels in brain, testis and ovary, followed by lung |
| Cellular Localization | Nucleus, nucleoplasm. Note=Punctate expression throughout the nucleoplasm and enriched in the perinucleolar region. Specifically nucleates at CpG islands where it's presence results in chromatin depleted in H3K36me2 |
| Entrez Gene | 22992 |
|---|
Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with JHDM1a/FBXL11 antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Western blot analysis of JHDM1a/FBXL11 (arrow) using rabbit polyclonal JHDM1a/FBXL11 Antibody (Center).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the JHDM1a/FBXL11 gene (Lane 2) (Origene Technologies).
Application
JHDM1a/FBXL11 Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the JHDM1a/FBXL11 antibody detected the JHDM1a/FBXL11 protein (arrow).| Application Notes | IHC:1:10~50 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
