Application
Western blot analysis of anti-JNK2 Pab in Jurkat cell lysate. JNK2 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with JNK2 antibody (C-term K373), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | JNK2 (MAPK9) Antibody (C-term K373) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MAPK9; JNK2; PRKM9; SAPK1A; Mitogen-activated protein kinase 9; JNK-55; Stress-activated protein kinase 1a; Stress-activated protein kinase JNK2; c-Jun N-terminal kinase 2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | JNK2 PRKM9 SAPK1A |
|---|---|
| Alternative Names | MAPK9 JNK2 PRKM9 SAPK1A Mitogen-activated protein kinase 9 JNK-55 Stress-activated protein kinase 1a Stress-activated protein kinase JNK2 c-Jun N-terminal kinase 2 |
| Molecular Weight(MW) | 48139 Da |
| Function | Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK9/JNK2 In turn, MAPK9/JNK2 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. In response to oxidative or ribotoxic stresses, inhibits rRNA synthesis by phosphorylating and inactivating the RNA polymerase 1-specific transcription initiation factor RRN3. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including TP53 and YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Upon T-cell receptor (TCR) stimulation, is activated by CARMA1, BCL10, MAP2K7 and MAP3K7/TAK1 to regulate JUN protein levels. Plays an important role in the osmotic stress-induced epithelial tight-junctions disruption. When activated, promotes beta-catenin/CTNNB1 degradation and inhibits the canonical Wnt signaling pathway Participates also in neurite growth in spiral ganglion neurons |
| Tissue Specificity | This JNK2 (MAPK9) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 366~396 amino acids from the C-terminal region of human JNK2. |
| Cellular Localization | Cytoplasm. Nucleus. |
| Entrez Gene | 5601 |
|---|
Application
Western blot analysis of anti-JNK2 Pab in Jurkat cell lysate. JNK2 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with JNK2 antibody (C-term K373), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
