Application
KLF9 Antibody (N-term) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the KLF9 antibody detected the KLF9 protein (arrow).Application
Fluorescent confocal image of U251 cell stained with KLF9 Antibody (N-term)(Cat#AP16249a).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with KLF9 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). KLF9 immunoreactivity is localized to vesicles and Nucleus significantly.| Product Name | KLF9 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KLF9; BTEB; BTEB1; Krueppel-like factor 9; GC-box-binding protein 1; Basic transcription element-binding protein 1; Transcription factor BTEB1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BTEB BTEB1 |
|---|---|
| Alternative Names | KLF9 BTEB BTEB1 Krueppel-like factor 9 GC-box-binding protein 1 Basic transcription element-binding protein 1 Transcription factor BTEB1 |
| Molecular Weight(MW) | 27235 Da |
| Function | Transcription factor that binds to GC box promoter elements. Selectively activates mRNA synthesis from genes containing tandem repeats of GC boxes but represses genes with a single GC box |
| Tissue Specificity | This KLF9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 28-57 amino acids from the N-terminal region of human KLF9. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 687 |
|---|---|
| Nucleotide Accession | NP_001197.1 |
Application
KLF9 Antibody (N-term) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the KLF9 antibody detected the KLF9 protein (arrow).
Application
Fluorescent confocal image of U251 cell stained with KLF9 Antibody (N-term)(Cat#AP16249a).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with KLF9 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). KLF9 immunoreactivity is localized to vesicles and Nucleus significantly.| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
