Application
Western blot analysis of anti-G9a Antibody (Center) in mouse testis tissue lysates (35ug/lane). G9a(arrow) was detected using the purified Pab .Application
G9a Antibody (C617) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the G9a antibody detected the G9a protein (arrow).| Product Name | KMT1C / G9a / BAT8 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EHMT2; BAT8; C6orf30; G9A; KMT1C; NG36; Histone-lysine N-methyltransferase EHMT2; Euchromatic histone-lysine N-methyltransferase 2; HLA-B-associated transcript 8; Histone H3-K9 methyltransferase 3; Lysine N-methyltransferase 1C; Protein G9a |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BAT8 C6orf30 G9A KMT1C NG36 |
|---|---|
| Alternative Names | EHMT2 BAT8 C6orf30 G9A KMT1C NG36 Histone-lysine N-methyltransferase EHMT2 Euchromatic histone-lysine N-methyltransferase 2 HLA-B-associated transcript 8 Histone H3-K9 methyltransferase 3 Lysine N-methyltransferase 1C Protein G9a |
| Molecular Weight(MW) | 132370 Da |
| Function | Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys- 373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself |
| Tissue Specificity | Expressed in all tissues examined, with high levels in fetal liver, thymus, lymph node, spleen and peripheral blood leukocytes and lower level in bone marrow |
| Cellular Localization | Nucleus. Chromosome. Note=Associates with euchromatic regions. Does not associate with heterochromatin |
| Entrez Gene | 10919 |
|---|
Application
Western blot analysis of anti-G9a Antibody (Center) in mouse testis tissue lysates (35ug/lane). G9a(arrow) was detected using the purified Pab .
Application
G9a Antibody (C617) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the G9a antibody detected the G9a protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
