Application
Western blot analysis of KRT1 Antibody (Center) in MCF-7 cell line lysates (35ug/lane). KRT1 (arrow) was detected using the purified Pab.Application
KRT1 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the KRT1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
KRT1 Antibody (Center) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | KRT1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KRT1; KRTA; Keratin, type II cytoskeletal 1; 67 kDa cytokeratin; Cytokeratin-1; Hair alpha protein; Keratin-1; Type-II keratin Kb1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KRTA |
|---|---|
| Alternative Names | KRT1 KRTA Keratin type II cytoskeletal 1 67 kDa cytokeratin Cytokeratin-1 Hair alpha protein Keratin-1 Type-II keratin Kb1 |
| Molecular Weight(MW) | 66039 Da |
| Function | May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein kinase C (RACK1/GNB2L1). In complex with C1QBP is a high affinty receptor for kininogen-1/HMWK |
| Tissue Specificity | The source of this protein is neonatal foreskin. The 67-kDa type II keratins are expressed in terminally differentiating epidermis |
| Cellular Localization | Cell membrane. Note=Located on plasma membrane of neuroblastoma NMB7 cells |
| Entrez Gene | 3848 |
|---|
Application
Western blot analysis of KRT1 Antibody (Center) in MCF-7 cell line lysates (35ug/lane). KRT1 (arrow) was detected using the purified Pab.
Application
KRT1 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the KRT1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
KRT1 Antibody (Center) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
