Application
Western blot analysis of anti-KSR1 Pab in A375 cell lysate. KSR1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | KSR1 Antibody (N-term A7) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KSR1; KSR; Kinase suppressor of Ras 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KSR |
|---|---|
| Alternative Names | KSR1 KSR Kinase suppressor of Ras 1 |
| Molecular Weight(MW) | 102032 Da |
| Function | Location-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. By itself, it has no demonstrated kinase activity |
| Tissue Specificity | This KSR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region with alanine at position 7 of human KSR1. |
| Cellular Localization | Cytoplasm. Membrane; Peripheral membrane protein (By similarity). Endoplasmic reticulum membrane. Note=In unstimulated cells, where the phosphorylated form is bound to a 14-3-3 protein, sequestration in the cytoplasm occurs. Following growth factor treatment, the protein is free for membrane translocation, and it moves from the cytoplasm to the cell periphery (By similarity) |
| Entrez Gene | 8844 |
|---|
Application
Western blot analysis of anti-KSR1 Pab in A375 cell lysate. KSR1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
