Application
Western blot analysis of Leo1-pS205 in CEM cell line lysates (35ug/lane). Leo1 (arrow) was detected using the purified Pab.Application
Western blot analysis of LEO1(arrow) using rabbit LEO1 Antibody (S205) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the LEO1 gene (Lane 2) (Origene Technologies).Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with LEO1 Antibody (S205) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | LEO1 Antibody (S205) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LEO1; RDL; RNA polymerase-associated protein LEO1; Replicative senescence down-regulated leo1-like protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | RDL |
|---|---|
| Alternative Names | LEO1 RDL RNA polymerase-associated protein LEO1 Replicative senescence down-regulated leo1-like protein |
| Molecular Weight(MW) | 75404 Da |
| Function | Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non- phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of MLL1; it promotes leukemogenesis though association with MLL-rearranged oncoproteins, such as MLL-MLLT3/AF9 and MLL-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Involved in polyadenylation of mRNA precursors. Connects PAF1C to Wnt signaling |
| Tissue Specificity | Highly expressed in skeletal muscle and heart. Weakly expressed in placenta and liver |
| Cellular Localization | Nucleus. |
Application
Western blot analysis of Leo1-pS205 in CEM cell line lysates (35ug/lane). Leo1 (arrow) was detected using the purified Pab.
Application
Western blot analysis of LEO1(arrow) using rabbit LEO1 Antibody (S205) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the LEO1 gene (Lane 2) (Origene Technologies).
Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with LEO1 Antibody (S205) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
