Application
LIMK1 (Thr508) antibody western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the LIMK1 antibody detected the LIMK1 protein (arrow).Application
LIMK1 antibody (Thr508) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the LIMK1 antibody (Thr508) for immunohistochemistry. Clinical relevance has not been evaluated.Application
LIMK1 Antibody (Thr508) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | LIMK1 (Thr508) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LIMK1; LIMK; LIM domain kinase 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | LIMK |
|---|---|
| Alternative Names | LIMK1 LIMK LIM domain kinase 1 |
| Molecular Weight(MW) | 72585 Da |
| Function | Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways Activated by upstream kinases including ROCK1, PAK1 and PAK4, which phosphorylate LIMK1 on a threonine residue located in its activation loop. LIMK1 subsequently phosphorylates and inactivates the actin binding/depolymerizing factors cofilin-1/CFL1, cofilin- 2/CFL2 and destrin/DSTN, thereby preventing the cleavage of filamentous actin (F-actin), and stabilizing the actin cytoskeleton. In this way LIMK1 regulates several actin-dependent biological processes including cell motility, cell cycle progression, and differentiation. Phosphorylates TPPP on serine residues, thereby promoting microtubule disassembly. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes |
| Tissue Specificity | Highest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle |
| Cellular Localization | Cytoplasm. Nucleus. Note=Predominantly found in the cytoplasm |
| Entrez Gene | 3984 |
|---|---|
| Nucleotide Accession | NP_002305.1 |
Application
LIMK1 (Thr508) antibody western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the LIMK1 antibody detected the LIMK1 protein (arrow).
Application
LIMK1 antibody (Thr508) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the LIMK1 antibody (Thr508) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
LIMK1 Antibody (Thr508) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
