Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using 14-3-3 beta antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE diluted at 1:10000Application
Sample (20 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:10000Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using 14-3-3 beta antibody at 1:200 dilution.| Product Name | 14-3-3 beta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to 14-3-3 beta (tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide)This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human 14-3-3 beta. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, Chicken, Rhesus Monkey, BovineRat (97%), Xenopus laevis (89%), Chicken (97%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | GW128 antibody HS1 antibody KCIP-1 antibody YWHAA antibody YWHAB antibody "brain protein 14-3-3 beta isoform antibody" protein 1054 antibody 14-3-3 protein beta/alpha antibody protein kinase C inhibitor protein-1 antibody "tyrosine 3-monooxygenas |
|---|---|
| Molecular Weight(MW) | 28 kDa (note) (3) |
| Cellular Localization | Cytoplasm , Melanosome |
Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using 14-3-3 beta antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE diluted at 1:10000
Application
Sample (20 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:10000
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using 14-3-3 beta antibody at 1:200 dilution.| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
