Application
Sample(30 μg of whole cell lysate) A:Raji 12% SDS PAGE diluted at 1:1000Application
Immunofluorescence analysis of methanol-fixed HeLa, using 14-3-3 beta antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using 14-3-3 beta antibody at 1:500 dilution.| Product Name | 14-3-3 beta antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to 14-3-3 beta (tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide)This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human 14-3-3 beta. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Rhesus Monkey, BovineMouse (100%), Rat (100%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | GW128 antibody HS1 antibody KCIP-1 antibody YWHAA antibody YWHAB antibody "brain protein 14-3-3 beta isoform antibody" protein 1054 antibody 14-3-3 protein beta/alpha antibody protein kinase C inhibitor protein-1 antibody "tyrosine 3-monooxygenas |
|---|---|
| Molecular Weight(MW) | 28 kDa (note) (2) |
Application
Sample(30 μg of whole cell lysate) A:Raji 12% SDS PAGE diluted at 1:1000
Application
Immunofluorescence analysis of methanol-fixed HeLa, using 14-3-3 beta antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using 14-3-3 beta antibody at 1:500 dilution.| Positive Control | 293T , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
