Application
Immunohistochemical analysis of paraffin-embedded A549 xenograft , using 14-3-3 epsilon antibody at 1:500 dilution.Application
Sample (30 µg of whole cell lysate) A: H1299 B: HeLa S3 12% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of methanol-fixed HeLa, using 14-3-3 epsilon antibody at 1:200 dilution.Application
Sample (20 µg of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
14-3-3 epsilon antibody [N1C3] immunoprecipitates YWHAE protein in IP experiments. IP samples: HeLa whole cell extract A. 40 μg HeLa whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of YWHAE protein by 4 μg 14-3-3 epsilon antibody [N1C3] 12 % SDS-PAGE The immunoprecipitated YWHAE protein was detected by 14-3-3 epsilon antibody [N1C3] diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]| Product Name | 14-3-3 epsilon antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to 14-3-3 epsilon (tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide)This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene. [provided by RefSeq] |
| Immunogen | Full length human 14-3-3 epsilon Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, Chicken, Chimpanzee, BovineRat (98%), Xenopus laevis (96%), Chicken (100%), Chimpanzee (100%), Bovine (100%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 14-3-3E antibody FLJ45465 antibody FLJ53559 antibody KCIP-1 antibody MDCR antibody MDS antibody YWHAE antibody "tyrosine 3/tryptophan 5 -monooxygenase activation protein epsilon polypeptide antibody" protein kinase C inhibitor protein-1 antibody |
|---|---|
| Molecular Weight(MW) | 29 kDa (note) |
| Cellular Localization | Cytoplasm , Melanosome |
Application
Immunohistochemical analysis of paraffin-embedded A549 xenograft , using 14-3-3 epsilon antibody at 1:500 dilution.
Application
Sample (30 µg of whole cell lysate) A: H1299 B: HeLa S3 12% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of methanol-fixed HeLa, using 14-3-3 epsilon antibody at 1:200 dilution.
Application
Sample (20 µg of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
14-3-3 epsilon antibody [N1C3] immunoprecipitates YWHAE protein in IP experiments. IP samples: HeLa whole cell extract A. 40 μg HeLa whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of YWHAE protein by 4 μg 14-3-3 epsilon antibody [N1C3] 12 % SDS-PAGE The immunoprecipitated YWHAE protein was detected by 14-3-3 epsilon antibody [N1C3] diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]| Positive Control | 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
